4O5N
Crystal structure of A/Victoria/361/2011 (H3N2) influenza virus hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2013-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.687, 100.687, 383.307 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.839 - 1.750 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.18470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yp7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.182 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.890 |
| Number of reflections | 75930 | |
| <I/σ(I)> | 21.2 | 2.4 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 8.9 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 277 | 5% PEG 8,000, 20% PEG 300, 10% glycerol, 0.1 M Tris pH 8.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
