4O59
Co-enzyme Induced Conformational Changes in Bovine Eye Glyceraldehyde 3-Phosphate Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.703, 125.273, 83.602 |
| Unit cell angles | 90.00, 118.02, 90.00 |
Refinement procedure
| Resolution | 47.800 - 1.520 |
| R-factor | 0.17977 |
| Rwork | 0.178 |
| R-free | 0.20904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j0x |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.062 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.520 |
| Rmerge | 0.094 |
| Number of reflections | 220468 |
| Completeness [%] | 98.5 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 20% PEG3350, 0.15 M malic acid pH 7.0, VAPOR DIFFUSION, temperature 277K |
