4O4S
Crystal structure of phycobiliprotein lyase CpcT complexed with phycocyanobilin (PCB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.746, 69.601, 162.595 |
| Unit cell angles | 90.21, 90.28, 60.12 |
Refinement procedure
| Resolution | 29.747 - 2.500 |
| R-factor | 0.1836 |
| Rwork | 0.180 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o4o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.516 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.058 |
| Number of reflections | 77314 |
| <I/σ(I)> | 20.5 |
| Completeness [%] | 83.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | MgCl2 6H2O (0.1M), Bis-Tris (0.1 M, pH 5.5) and polyethylene glycol 3350 (14% w/v), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
