4O4K

DNA Double-Strand Break Repair Pathway Choice Is Directed by Distinct MRE11 Nuclease Activities

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	47.769, 113.154, 80.891
Unit cell angles	90.00, 101.47, 90.00

Refinement procedure

Resolution	37.409 - 2.100
R-factor	0.1955
Rwork	0.193
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nzv
RMSD bond length	0.008
RMSD bond angle	1.237
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	46357
Completeness [%]	94.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	288	pH 6, VAPOR DIFFUSION, temperature 288K