4O48
Crystal structure of cleaved guinea pig L-asparaginase type III in complex with L-aspartate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.580, 114.580, 138.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.650 - 2.290 |
| R-factor | 0.18447 |
| Rwork | 0.182 |
| R-free | 0.22110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.498 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Number of reflections | 47199 | |
| <I/σ(I)> | 14.88 | 3.19 |
| Completeness [%] | 98.7 | 95.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M MES pH 6.0, 0.2 M ammonium sulfate, 13-15% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
