4O47
Crystal structure of uncleaved guinea pig L-asparaginase type III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.872, 114.872, 138.971 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.730 - 1.900 |
| R-factor | 0.19413 |
| Rwork | 0.193 |
| R-free | 0.21337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GDV |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.575 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 83640 | |
| <I/σ(I)> | 14.75 | 2.59 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M MES pH 6.0, 0.2 M ammonium sulfate, 13-15% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
