4O46
14-3-3-gamma in complex with influenza NS1 C-terminal tail phosphorylated at S228
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 121.829, 121.829, 314.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.530 - 2.900 |
R-factor | 0.2262 |
Rwork | 0.226 |
R-free | 0.24940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uzd |
RMSD bond length | 0.008 |
RMSD bond angle | 0.910 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 113.590 | 38.526 | 3.060 |
High resolution limit [Å] | 1.800 | 9.170 | 1.800 |
Rmerge | 0.028 | 0.920 | |
Total number of observations | 24449 | 115226 | |
Number of reflections | 53464 | ||
<I/σ(I)> | 21.7 | 20.3 | 0.8 |
Completeness [%] | 100.0 | 98.7 | 100 |
Redundancy | 14.8 | 12.9 | 15.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 291 | 20% PEG3350, 0.2 M magnesium nitrate, temperature 291K |