4O41
Amide linked RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-08-17 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.7686 |
| Spacegroup name | P 1 |
| Unit cell lengths | 22.360, 34.330, 43.300 |
| Unit cell angles | 110.03, 94.24, 95.83 |
Refinement procedure
| Resolution | 28.000 - 1.200 |
| Rwork | 0.147 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.033 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Single (wavelength anomalous dispersion) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.400 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.076 | 0.296 |
| Number of reflections | 35934 | |
| <I/σ(I)> | 14.66 | 6.21 |
| Completeness [%] | 95.8 | 94.8 |
| Redundancy | 7.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 20 mM sodium cacodylate, 45 mM strontium chloride, 10 mM magnesium chloride, 6 mM spermine tetrahydrochloride and 5% 2-methyl-2,4-pentanediol [MPD (v/v)], pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
