4O3W

Crystal structure of the vaccine antigen Transferrin Binding Protein B (TbpB) mutant Tyr-63-Ala from Actinobacillus suis H57

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-17
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	90.810, 74.510, 106.600
Unit cell angles	90.00, 105.80, 90.00

Refinement procedure

Resolution	46.279 - 2.100
R-factor	0.1907
Rwork	0.190
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.123
Data scaling software	XSCALE
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.279
High resolution limit [Å]	2.100
Number of reflections	74646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M Bis-Tris, 0.4 M lithium sulfate, 17% PEG 3350, 20% glycerol, pH 5.5, vapor diffusion, hanging drop, temperature 293K