4O36
Semisynthetic RNase S1-15-H7/11-Q10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 43.910, 43.910, 96.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.870 - 1.220 |
| R-factor | 0.18547 |
| Rwork | 0.184 |
| R-free | 0.22599 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rns |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.315 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.030 |
| High resolution limit [Å] | 1.220 |
| Number of reflections | 32726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 292 | 100 mM sodium citrate 2.4 M ammonium sulfate, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
