4O34
Room temperature macromolecular serial crystallography using synchrotron radiation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 296 |
Detector technology | PIXEL |
Collection date | 2013-08-23 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.27 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.300, 79.300, 38.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.070 - 2.090 |
R-factor | 0.18152 |
Rwork | 0.176 |
R-free | 0.23011 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.076 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.070 | 2.140 |
High resolution limit [Å] | 1.700 | 2.090 |
Number of reflections | 16081 | |
<I/σ(I)> | 8.1 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 1755 | 1281 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | batch | 3 | 293 | 14.7 % (w/v) NaCl, 22 % (w/v) PEG 8000, 500 mM acetate buffer pH 3, 100 mg/ml lysozyme, batch, temperature 293K |