4O32

Structure of a malarial protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	200
Detector technology	CCD
Collection date	2011-04-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97965
Spacegroup name	P 43 21 2
Unit cell lengths	77.030, 77.030, 134.415
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.642 - 2.196
R-factor	0.2094
Rwork	0.207
R-free	0.24860
Structure solution method	SAD
Starting model (for MR)	SAD derived from 3UL3
RMSD bond length	0.004
RMSD bond angle	0.696
Data reduction software	XDS
Data scaling software	XDS
Phasing software	HKL2Map
Refinement software	PHENIX ((phenix.refine: dev_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.642
High resolution limit [Å]	2.196
Rmerge	0.047
Number of reflections	21285
<I/σ(I)>	22.9
Completeness [%]	99.6
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 4000, 100 mM citrate, isopropanol 10-15%, VAPOR DIFFUSION, HANGING DROP, temperature 298K