4O26
Crystal structure of the TRBD domain of TERT and the CR4/5 of TR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 118.092, 118.092, 358.021 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.971 - 3.001 |
| R-factor | 0.2229 |
| Rwork | 0.221 |
| R-free | 0.26090 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.018 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.096 | 0.049 | 0.568 |
| Number of reflections | 30608 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 99.9 | 98.9 | 100 |
| Redundancy | 8.2 | 7.2 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 100 mM sodium acetate, pH 4.5, 1 M ammonium sulfate, 0.3 M sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
