4O1W
Crystal Structure of Colwellia psychrerythraea cytochrome c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-12 |
Detector | MAR CCD 165 mm |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.280, 45.043, 87.574 |
Unit cell angles | 90.00, 90.85, 90.00 |
Refinement procedure
Resolution | 19.950 - 2.000 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cno |
RMSD bond length | 0.017 |
RMSD bond angle | 1.527 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.950 | 19.950 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 34186 | |
Completeness [%] | 99.1 | 92.2 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 293 | 30% PEG 4000, 0.2M lithium sulfate, 50mM Tris, 5% trehalose, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |