4O1R
Crystal structure of NpuDnaB intein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 61.054, 61.054, 122.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.050 - 1.400 |
| R-factor | 0.16179 |
| Rwork | 0.161 |
| R-free | 0.18906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mi8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.288 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.050 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.081 | 0.932 |
| Number of reflections | 46443 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-Tris, pH 5.5, 3.0 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
