4O1M
Toxoplasma gondii Enoyl acyl carrier protein reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.400, 75.570, 188.330 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.17022 |
| Rwork | 0.169 |
| R-free | 0.19949 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb 202s |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.541 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.500 |
| Number of reflections | 129052 | |
| <I/σ(I)> | 6.5 | 2.8 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 0.1M Na-Formate, NH4-Acetate, Na3-Citrate, NaK-Tartrate, Na-Oxamate, 1M Sodium HEPES, MOPS pH7.5, 30%(v/v) P500MME_P20K), VAPOR DIFFUSION, SITTING DROP, temperature 290K |
