4O0Q
Apo structure of a methyltransferase component involved in O-demethylation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2013-05-23 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 90.670, 119.070, 58.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.690 - 1.920 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.913 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.700 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 48906 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 277.15 | 100mM Tris pH 7.5, 100mM succinic acid, 15%(v/v) PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
