4O0M

Crystal structure of T. Elongatus BP-1 Clock Protein KaiC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-28
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	C 2 2 21
Unit cell lengths	130.810, 195.290, 136.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.995 - 2.840
R-factor	0.2271
Rwork	0.225
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.807
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.000	2.900
High resolution limit [Å]	2.840	2.840
Rmerge	0.110	0.769
Number of reflections	79142
<I/σ(I)>	9.6	1.8
Completeness [%]	99.1	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	291	Acetate buffer and 1M sodium formate, pH 4.5, VAPOR DIFFUSION, temperature 291K