4O0L
Crystal structure of NADPH-Dependent 3-Quinuclidinone Reductase from Rhodotorula Rubra
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Detector technology | CCD |
Collection date | 2008-03-04 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.272, 91.272, 265.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.1844 |
Rwork | 0.182 |
R-free | 0.22260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vl8 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.517 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 55859 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10 | 278 | 100MM CHES, 30% PEG8000, 3% SUCROSE, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K |