4O0H
Crystal structure of human L-asparaginase protein with covalently linked substrate L-asparagine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2012-05-25 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 |
Unit cell lengths | 59.651, 59.651, 298.517 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.830 - 1.970 |
R-factor | 0.16881 |
Rwork | 0.166 |
R-free | 0.23139 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4GDV |
RMSD bond length | 0.011 |
RMSD bond angle | 1.655 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.090 |
High resolution limit [Å] | 1.970 | 1.970 |
Number of reflections | 38615 | |
<I/σ(I)> | 9.16 | 2.05 |
Completeness [%] | 91.9 | 80.5 |
Redundancy | 3.36 | 3.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.2-2.5 M sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |