4O0G
Crystal structure of the human L-asparaginase protein T219V mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0782 |
Spacegroup name | P 65 |
Unit cell lengths | 59.439, 59.439, 306.794 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.180 - 2.100 |
R-factor | 0.16867 |
Rwork | 0.165 |
R-free | 0.23620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4GDV |
RMSD bond length | 0.014 |
RMSD bond angle | 1.766 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.220 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 34954 | |
<I/σ(I)> | 11.54 | 2.79 |
Completeness [%] | 97.9 | 96 |
Redundancy | 3.6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.2-2.5 M sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |