4O0D
Crystal structure of the human L-asparaginase protein T168S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 59.700, 59.700, 299.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.270 - 1.950 |
| R-factor | 0.17803 |
| Rwork | 0.175 |
| R-free | 0.23401 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GDV |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.591 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.106 | 0.523 |
| Number of reflections | 36456 | |
| <I/σ(I)> | 11.75 | 3.18 |
| Completeness [%] | 82.6 | 71.4 |
| Redundancy | 5.89 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.2-2.5 M sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
