4O0C
High resolution crystal structure of uncleaved human L-asparaginase protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 65 |
| Unit cell lengths | 59.213, 59.213, 297.793 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.040 - 1.500 |
| R-factor | 0.15465 |
| Rwork | 0.152 |
| R-free | 0.20529 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GDV |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.559 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 92426 | |
| <I/σ(I)> | 13.2 | 2.86 |
| Completeness [%] | 99.0 | 94.3 |
| Redundancy | 8.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.2-2.5 M sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
