4O06
1.15A Resolution Structure of the Proteasome Assembly Chaperone Nas2 PDZ Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 39.968, 39.968, 115.827 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.895 - 1.150 |
R-factor | 0.1727 |
Rwork | 0.172 |
R-free | 0.19400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rle |
RMSD bond length | 0.012 |
RMSD bond angle | 1.356 |
Data scaling software | Aimless (0.2.8) |
Phasing software | BALBES |
Refinement software | PHENIX (dev_1563) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.970 | 1.170 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.077 | 0.107 |
Total number of observations | 27697 | |
Number of reflections | 33545 | |
<I/σ(I)> | 23 | 3.4 |
Completeness [%] | 98.2 | 95.1 |
Redundancy | 12.5 | 17.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 293 | 10% (w/v) PEG 2000MME, 100 mM sodium acetate, 200 mM ammonium sulfate, pH 5.5, vapor diffusion, temperature 293K |