4O06

1.15A Resolution Structure of the Proteasome Assembly Chaperone Nas2 PDZ Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	39.968, 39.968, 115.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.895 - 1.150
R-factor	0.1727
Rwork	0.172
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rle
RMSD bond length	0.012
RMSD bond angle	1.356
Data scaling software	Aimless (0.2.8)
Phasing software	BALBES
Refinement software	PHENIX (dev_1563)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.970	1.170
High resolution limit [Å]	1.150	1.150
Rmerge	0.077	0.107
Total number of observations		27697
Number of reflections	33545
<I/σ(I)>	23	3.4
Completeness [%]	98.2	95.1
Redundancy	12.5	17.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	10% (w/v) PEG 2000MME, 100 mM sodium acetate, 200 mM ammonium sulfate, pH 5.5, vapor diffusion, temperature 293K