4O01
Crystal Structure of D. radiodurans Bacteriophytochrome Photosensory Core Module in its Illuminated Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98043 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.900, 195.700, 225.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.220 - 3.240 |
R-factor | 0.234 |
Rwork | 0.233 |
R-free | 0.25972 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o9c |
RMSD bond length | 0.005 |
RMSD bond angle | 1.159 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.220 | 3.320 | |
High resolution limit [Å] | 3.240 | 15.000 | 3.240 |
Rmerge | 0.241 | 0.105 | 4.390 |
Number of reflections | 61928 | ||
<I/σ(I)> | 9.69 | 27.21 | 0.83 |
Completeness [%] | 99.9 | 95.8 | 100 |
Redundancy | 19.31 | 16.5 | 20.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris, 0.2M NaCl, 25% PEG 3350, sequential 655nm illumination, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |