4NZT
Crystal structure of the antibody-binding region of Protein M (Protein M TD) in complex with anti-infleunza hemagglutinin antibody CR9114 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.865, 91.468, 126.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.727 - 2.497 |
R-factor | 0.1809 |
Rwork | 0.177 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 4NZR AND 4FQH |
RMSD bond length | 0.007 |
RMSD bond angle | 1.117 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.497 | 2.497 |
Number of reflections | 27816 | |
<I/σ(I)> | 15.3 | 1.5 |
Completeness [%] | 98.0 | 84.5 |
Redundancy | 5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.29 | 295 | 0.1 M MES, pH 6.26, 6% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 295K |