4NZS

Crystal structure of beta-ketothiolase BktB B from Ralstonia eutropha H16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-20
Detector	Quantum 270
Wavelength(s)	0.97985
Spacegroup name	C 2 2 21
Unit cell lengths	106.954, 107.236, 144.137
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.740 - 2.290
R-factor	0.2654
Rwork	0.253
R-free	0.31890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dlu
RMSD bond length	0.014
RMSD bond angle	1.732
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	35.740
High resolution limit [Å]	2.290
Number of reflections	31979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	PEG3350, Li2SO4, pH 6.5, vapor diffusion, hanging drop, temperature 295K