4NZS
Crystal structure of beta-ketothiolase BktB B from Ralstonia eutropha H16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-20 |
Detector | Quantum 270 |
Wavelength(s) | 0.97985 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 106.954, 107.236, 144.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.740 - 2.290 |
R-factor | 0.2654 |
Rwork | 0.253 |
R-free | 0.31890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dlu |
RMSD bond length | 0.014 |
RMSD bond angle | 1.732 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.740 |
High resolution limit [Å] | 2.290 |
Number of reflections | 31979 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG3350, Li2SO4, pH 6.5, vapor diffusion, hanging drop, temperature 295K |