4NZP

The crystal structure of argininosuccinate synthase from Campylobacter jejuni subsp. jejuni NCTC 11168

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	I 2 2 2
Unit cell lengths	64.646, 97.360, 141.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.571 - 2.307
R-factor	0.183
Rwork	0.180
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1J1Z
RMSD bond length	0.007
RMSD bond angle	1.044
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300
Rmerge	0.138
Number of reflections	19759
<I/σ(I)>	16.7
Completeness [%]	98.5
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.1M HEPES, 10% (w/v) PEG6000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K