4NZI

Crystal structure of murine neuroglobin mutant V140W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.86437
Spacegroup name	H 3 2
Unit cell lengths	87.214, 87.214, 114.399
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.599 - 2.100
R-factor	0.2233
Rwork	0.219
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.266
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	2.175
High resolution limit [Å]	2.100	2.100
Number of reflections	9964
Completeness [%]	99.7	97.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.6M ammonium sulphate, 0.1M MES, 10% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K