4NZG
Crystal Structure of the N-terminal domain of Moloney murine leukemia virus integrase, Northeast Structural Genomics Consortium Target OR3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07500 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.659, 38.440, 135.275 |
| Unit cell angles | 90.00, 91.70, 90.00 |
Refinement procedure
| Resolution | 45.072 - 2.152 |
| R-factor | 0.2359 |
| Rwork | 0.234 |
| R-free | 0.26540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nnq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.795 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 135.220 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.081 | 0.605 |
| Number of reflections | 24941 | |
| <I/σ(I)> | 2.46 | |
| Completeness [%] | 98.1 | 97 |
| Redundancy | 6.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution:1.0 M K2HPO4, 0.1 M NaAc, 0.05% Anapoe X-305, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
