4NZD

Interleukin 21 receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.04
Spacegroup name	I 2 2 2
Unit cell lengths	114.330, 125.260, 178.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.778 - 2.750
R-factor	0.2407
Rwork	0.239
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.142
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.780	2.848
High resolution limit [Å]	2.750	2.750
Number of reflections	33261
Completeness [%]	99.0	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	278	5 mg/ml Protein in 10 mM Hepes, 150 mM NaCl, pH7.5 Crystalized in 1M Potassium sodium tartrate tetrahydrate, 3% W/V Peg5000 and 0.1 M tris Ph 8.5. Drops were 1:1, VAPOR DIFFUSION, SITTING DROP, temperature 278K