4NYR

In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-binding, lipocalin-like protein

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	70
Detector technology	PIXEL
Collection date	2013-05-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	2.7
Spacegroup name	P 1
格子定数 [Å]	32.280, 33.220, 40.180
格子定数 [度]	99.50, 100.28, 104.11

精密化法

分解能	38.580 - 2.490
R因子	0.16159
Rwork	0.154
R-free	0.23262
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	1.906
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.7.0032)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.560
分解能 [Å] (高)	2.490	2.490
独立反射数	5421
<I/σ(I)>	38.07	22.43
完全性 [%]	99.6	96.7
冗長性	20

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	In-vivo crystallization	6	300	Spontaneous crystallization inside the midguts of a living viviparous cockroach, pH 6.0, In-vivo crystallization, temperature 300K