4NYR
In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-binding, lipocalin-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 70 |
| Detector technology | PIXEL |
| Collection date | 2013-05-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 2.7 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.280, 33.220, 40.180 |
| Unit cell angles | 99.50, 100.28, 104.11 |
Refinement procedure
| Resolution | 38.580 - 2.490 |
| R-factor | 0.16159 |
| Rwork | 0.154 |
| R-free | 0.23262 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.906 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 5421 | |
| <I/σ(I)> | 38.07 | 22.43 |
| Completeness [%] | 99.6 | 96.7 |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | In-vivo crystallization | 6 | 300 | Spontaneous crystallization inside the midguts of a living viviparous cockroach, pH 6.0, In-vivo crystallization, temperature 300K |
