4NYN

Crystal structure of RNase H1 from halophilic archaeon Halobacterium salinarum NRC-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-10
Detector	BRUKER SMART 6500
Wavelength(s)	0.9
Spacegroup name	I 41
Unit cell lengths	115.802, 115.802, 38.282
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.940 - 1.410
R-factor	0.1767
Rwork	0.175
R-free	0.20560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ehg
RMSD bond length	0.008
RMSD bond angle	1.026
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.430
High resolution limit [Å]	1.410	3.830	1.410
Rmerge	0.074	0.044	0.381
Number of reflections	48997
<I/σ(I)>	6.6
Completeness [%]	99.9	99.1	98.9
Redundancy	7.4	7.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	20%(W/V) PEG 8000, Imidazole(pH6.5), 3%(V/V) MPD, 10mM Manganese(II) chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K