4NY5
X-ray structure of the adduct formed between hen egg white lysozyme and NAMI-A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-10 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.772, 77.772, 37.316 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.150 - 1.850 |
R-factor | 0.17933 |
Rwork | 0.176 |
R-free | 0.23994 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4j1a |
RMSD bond length | 0.018 |
RMSD bond angle | 1.768 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 22.150 |
High resolution limit [Å] | 1.850 |
Rmerge | 0.095 |
Number of reflections | 10143 |
<I/σ(I)> | 5.3 |
Completeness [%] | 98.9 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | Crystals of the HEWL/NAMI-A adduct suitable for X-ray diffraction studies Have been grown by hanging drop vapor diffusion by mixing 1 L of 20 mg/mL HEWL/NAMI-A complex (protein to metal ratio 1:10) with an equal volume of reservoir solution containing 1.0 M NaCl and 50 mM sodium acetate pH 4.5. These crystals were fished with nylon loops and flash-frozen at 100 K using nitrogen gas, without cryoprotectant. This procedure partly dehydrates the crystals, enhancing in some cases the resolution of X-ray diffraction data, VAPOR DIFFUSION, HANGING DROP, temperature 293K |