4NXX
Crystal structure of the cytosolic domain of human MiD51
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.45860 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.716, 57.716, 255.398 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.816 - 2.550 |
Rwork | 0.208 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.761 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.086 | 0.499 |
Number of reflections | 15007 | |
<I/σ(I)> | 31.2 | 7.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 25.9 | 27.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.0M sodium malonate, 0.01M GDP, 0.01M manganese chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |