4NXU

Crystal structure of the cytosolic domain of human MiD51

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	P 1
Unit cell lengths	72.573, 79.265, 79.375
Unit cell angles	65.44, 84.18, 63.35

Refinement procedure

Resolution	39.189 - 2.300
Rwork	0.216
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.028
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.082	0.489
Number of reflections	62135
<I/σ(I)>	10.8	2.7
Completeness [%]	97.8
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	16% PEG 8000, 0.1M HEPES, 10% 2-propanol, 0.2M ammonium sulfate, 0.01M ADP, 0.01M manganese chloride , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K