4NXK
Crystal structure of Abp-D197A, a catalytic mutant of a GH27-b-L-arabinopyranosidase from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.701, 203.525, 286.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.860 - 2.300 |
| R-factor | 0.17109 |
| Rwork | 0.170 |
| R-free | 0.20069 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nx0 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.841 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.860 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.086 | |
| Number of reflections | 255755 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 97.1 | 96.32 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.8M ammonium sulfate, 0.1M citrate buffer, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
