4NXC
SPERM WHALE MYOGLOBIN UNDER 30 BAR NITROUS Oxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2009-10-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.449, 30.545, 63.634 |
| Unit cell angles | 90.00, 104.57, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| R-factor | 0.19141 |
| Rwork | 0.190 |
| R-free | 0.22455 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jho |
| RMSD bond length | 0.010 |
| RMSD bond angle | 3.040 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.630 | |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 18602 | |
| <I/σ(I)> | 16.8 | 4.5 |
| Completeness [%] | 95.3 | 93.9 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 7 | 300 | 50mM potassium phosphate, saturated ammonium sulphate., pH 7, BATCH, temperature 300K |
