4NX9
Crystal structure of Pseudomonas aeruginosa flagellin FliC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-03 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.347, 60.267, 46.611 |
| Unit cell angles | 90.00, 111.18, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.16357 |
| Rwork | 0.161 |
| R-free | 0.20493 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.555 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol (of Phenix) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.127 | 0.427 |
| Number of reflections | 14698 | |
| <I/σ(I)> | 20.8 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 22%(w/v) PEG6000, 0.1M bicine, 8% 2,2,2-trifluoro-ethanol, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
