4NWV
Crystal structure of Orsay virus-like particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.1271 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 402.197, 369.862, 410.511 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.250 |
| R-factor | 0.2778 |
| Rwork | 0.278 |
| R-free | 0.27890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RAVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
| High resolution limit [Å] | 3.250 | 8.810 | 3.250 |
| Rmerge | 0.190 | 0.038 | 0.720 |
| Number of reflections | 410231 | ||
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 87.8 | 94.5 | 58.3 |
| Redundancy | 3 | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.7-1.05 M sodium malonate, 0.25%-1% (v/v) Jeffamine ED-2001, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
