4NWE
Lysozyme UNDER 30 BAR PRESSURE OF NITROUS OXIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2009-05-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.188, 79.188, 37.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.580 |
| R-factor | 0.17102 |
| Rwork | 0.170 |
| R-free | 0.19994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c10 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.325 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 17064 | |
| <I/σ(I)> | 41.3 | 4.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 4.6 | 300 | 0.1M sodium acetate, 0.6-1.1M NaCl, pH 4.6, BATCH, temperature 300K |
