4NWB
Crystal structure of Mrt4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979214 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.292, 65.271, 71.404 |
| Unit cell angles | 90.00, 111.03, 90.00 |
Refinement procedure
| Resolution | 43.020 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20550 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.026 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.023 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.062 | 0.550 |
| Number of reflections | 23803 | |
| <I/σ(I)> | 17.8 | 3.5 |
| Completeness [%] | 97.7 | 96.8 |
| Redundancy | 7.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 0.1 M bicine, 2.22 M (NH4)2SO4, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
