4NW4
Crystal structure of a DUF4822 family protein (EF0375) from Enterococcus faecalis V583 at 1.85 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-21 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 |
Unit cell lengths | 61.379, 61.379, 160.389 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.731 - 1.850 |
R-factor | 0.1512 |
Rwork | 0.150 |
R-free | 0.18150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.384 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 26.731 | 26.731 | 1.900 |
High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
Rmerge | 0.013 | 0.013 | |
Total number of observations | 2526 | 14866 | |
Number of reflections | 29118 | ||
<I/σ(I)> | 9.4 | 16.5 | 0.6 |
Completeness [%] | 99.9 | 97.3 | 99.9 |
Redundancy | 6.6 | 7.4 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 2.400M ammonium sulfate, 0.1M TRIS pH 8.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |