4NV6
C212A mutant of Synechococcus VKOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-10-22 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 61 |
Unit cell lengths | 139.563, 139.563, 68.724 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.683 - 4.190 |
R-factor | 0.3022 |
Rwork | 0.299 |
R-free | 0.33110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.842 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PARROT |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.270 |
High resolution limit [Å] | 4.190 | 4.190 |
Rmerge | 0.111 | 0.949 |
Number of reflections | 5732 | |
<I/σ(I)> | 16.4 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.5 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | A 20mg/ml protein solution was mixed overnight with 15mg/ml DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), and 0.5% DDM. This mixture was crystallized with a buffer containing 11% PEG1500, 8% glycerol, 5% ethanol, 0.1M MgCl2, 0.1M NaCl, and 0.1M sodium cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |