4NUU
Heterotrimer structure of Region II from Plasmodium vivax Duffy Binding Protein (PvDBP) bound to the ectodomain of the Duffy Antigen Receptor for Chemokines (DARC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2012-02-10 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.9792909 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.590, 66.990, 97.920 |
| Unit cell angles | 90.00, 102.11, 90.00 |
Refinement procedure
| Resolution | 19.863 - 1.950 |
| R-factor | 0.1665 |
| Rwork | 0.165 |
| R-free | 0.20150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.743 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 55096 | |
| Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 290 | 0.1 M HEPES and 20% (w/v) polyethylene glycol 6000, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 7.4 |
