4NUT
Crystal structure of the complex between Snu13p and the PEP domain of Rsa1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.732, 59.732, 92.549 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.580 - 1.550 |
| R-factor | 0.19831 |
| Rwork | 0.196 |
| R-free | 0.23507 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.206 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 24971 | |
| Completeness [%] | 99.8 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 2.3M ammonium sulfate, 70mM sodium citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
