4NUR
Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2012-06-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97930 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.780, 76.656, 103.178 |
Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
Resolution | 39.749 - 1.761 |
R-factor | 0.1538 |
Rwork | 0.152 |
R-free | 0.18400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.084 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.760 |
Number of reflections | 137186 |
Completeness [%] | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.05M Magnesium acetate, 20%(v/w) PEG4000, pH 4.5, vapor diffusion, sitting drop, temperature 293K |