4NU2

Crystal structure of an ice-binding protein (FfIBP) from the Antarctic bacterium, Flavobacterium frigoris PS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 41 2 2
Unit cell lengths	69.171, 69.171, 178.764
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.810 - 2.100
R-factor	0.21005
Rwork	0.208
R-free	0.23901
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uyu
RMSD bond length	0.022
RMSD bond angle	2.047
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.810	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	24497
Completeness [%]	97.8	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	293	0.1M sodium acetate, 3M sodium chloride, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K