4NTS
Apo structure of the catalytic subunit of cAMP-dependent protein kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Detector technology | CCD |
| Collection date | 2011-12-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.960, 142.610, 63.160 |
| Unit cell angles | 90.00, 103.65, 90.00 |
Refinement procedure
| Resolution | 56.380 - 2.900 |
| R-factor | 0.24675 |
| Rwork | 0.244 |
| R-free | 0.29185 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j3h |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.013 |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 61.380 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 19014 |
| Completeness [%] | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277.15 | 8% MPD, 0.1 M Bicine, 150 mM Ammonium Acetate, 10 mM DTT, 9% methanol added to the well solution, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
