4NTL

Crystal structure of a lipoprotein, YaeC family (EF3198) from Enterococcus faecalis V583 at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-17
Detector	ADSC Q315
Wavelength(s)	0.979347,0.918401,0.979261
Spacegroup name	I 4 2 2
Unit cell lengths	97.161, 97.161, 144.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.013 - 1.799
R-factor	0.1807
Rwork	0.179
R-free	0.21260
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.547
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.013	29.013	1.850
High resolution limit [Å]	1.799	8.050	1.799
Rmerge		0.019	0.019
Total number of observations		5160	32237
Number of reflections	32421
<I/σ(I)>	11.3	11	0.3
Completeness [%]	100.0	97.9	100
Redundancy	13.8	12.3	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6	277	0.2000M zinc acetate, 10.0000% polyethylene glycol 8000, 0.1M MES pH 6.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K